作 者：Shengnan Zhai, Genying Li, Youwei Sun, Jianmin Song, Jihu Li, Guoqi Song, Yulian Li, Hongqing Ling, Zhonghu HeEmail author and Xianchun Xia

　　影响因子: 3.631

　　刊物名称: BMC Plant Biology

　　出版年份: 2016

　　卷: 16   期:1   页码: 1-15

　　doi: 10.1186/s12870-016-0916-z

　　文章摘要 : 

　　Phytoene synthase 1 (PSY1) is the most important regulatory enzyme in carotenoid biosynthesis, whereas its function is hardly known in common wheat. The aims of the present study were to investigate Psy1 function and genetic regulation using reverse genetics approaches.Transcript levels of Psy1 in RNAi transgenic lines were decreased by 54–76 % and yellow pigment content (YPC) was reduced by 26–35 % compared with controls, confirming the impact of Psy1 on carotenoid accumulation. A series of candidate genes involved in secondary metabolic pathways and core metabolic processes responded to Psy1 down-regulation. The aspartate rich domain (DXXXD) was important for PSY1 function, and conserved nucleotides adjacent to the domain influenced YPC by regulating gene expression, enzyme activity or alternative splicing. Compensatory responses analysis indicated that three Psy1 homoeologs may be coordinately regulated under normal conditions, but separately regulated under stress. The period 14 days post anthesis (DPA) was found to be a key regulation node during grain development.The findings define key aspects of flour color regulation in wheat and facilitate the genetic improvement of wheat quality targeting color/nutritional specifications required for specific end products.

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