作 者：Cuilan Shi, Yulong Ren, Linglong Liu, Fan Wang, Huan Zhang, Peng Tian, Tian Pan, Yongfei Wang, Ruonan Jing, Tianzhen Liu, Fuqing Wu, Qibing Lin, Cailin Lei, Xin Zhang, Shanshan Zhu, Xiuping Guo, Jiulin Wang, Zhichao Zhao, Jie Wang, Huqu Zhai, Zhijun Cheng, Jianmin Wan

　　影响因子：7.433

　　刊物名称：New Phytologist

　　出版年份：February 2019

　　doi：10.1104/pp.19.00065

　　文章摘要：Ubiquitination and deubiquitination are reversible processes that play crucial roles in regulating organ size in plants. However, information linking deubiquitination and seed size in rice is limited. Here we characterized a dominant large grain mutant lg1-D (large grain 1-D) with a 30.8% increase in seed width and 34.5% increase in 1000-grain weight relative to the wild type. The lg1-D mutant had more cells oriented in the lateral direction of the spikelet hull compared to the wild type. Map-based cloning showed that LG1 encodes a constitutively expressed ubiquitin-specific protease 15 (OsUBP15) that possesses de-ubiquitination activity in vitro. Loss-of-function and down-regulated expression of OsUBP15 produced narrower and smaller grains than the control. A set of in vivo experiments indicated that the mutant Osubp15 had enhanced protein stability relative to wild-type OsUBP15. Further experiments verified that OsDA1 directly interacted with OsUBP15. Genetic data indicated that OsUBP15 and GW2 were not independent in regulating grain width and size. In summary, we identified OsUBP15 as a positive regulator of grain width and size in rice and provide a promising strategy for improvement of grain yield by pyramiding OsUBP15 and gw2.

　　下载链接：http://www.plantphysiol.org/content/early/2019/02/22/pp.19.00065