Dong Huixue,Li Danping,Yang Ruizhen,Zhang Lichao,Zhang Yunwei,Liu Xu,Kong Xiuying,Sun Jiaqiang.

Plant Cell,2023,IF 11.60

DOI:10.1093/plcell/koad090

ABSTRACT:

GSK3 phosphorylates the Green Revolution protein Rht-B1b, enhancing its ability to inhibit target transcription factor activity to reduce plant height in wheat. The utilization of stabilized DELLA proteins Rht-B1b and Rht-D1b was crucial for increasing wheat (Triticum aestivum) productivity during the Green Revolution. However, the underlying mechanisms remain to be clarified. Here, we cloned a gain-of-function allele of the GSK3/SHAGGY-like kinase-encoding gene GSK3 by characterizing a dwarf wheat mutant. Furthermore, we determined that GSK3 interacts with and phosphorylates the Green Revolution protein Rht-B1b to promote it to reduce plant height in wheat. Specifically, phosphorylation by GSK3 may enhance the activity and stability of Rht-B1b, allowing it to inhibit the activities of its target transcription factors. Taken together, we reveal a positive regulatory mechanism for the Green Revolution protein Rht-B1b by GSK3, which might have contributed to the Green Revolution in wheat.