作 者：Wang Kunyang, Lin Zhishan, Wang Long, Wang Ke, Shi Qinghua, Du Lipu, Ye Xingguo*

　　影响因子：4.132

　　刊物名称：Theoretic and Applied Genetics

　　出版年份：2018

　　卷：131  期：1   页码：13-25

　　doi：10.1007/s00122-017-2982-5

　　文章摘要 : 

　　Aegilops longissimais an important wild species of common wheat that harbors many genes that can be used to improve various traits of common wheat (Triticum aestivum L.). To efficiently transfer the traits conferred by these Ae. longissima genes into wheat, we sequenced the whole expression transcript of Ae. longissima. Using the transcriptome data, we developed 134 specific polymerase chain reaction (PCR) markers located on the 14 chromosome arms of Ae. longissima. These novel molecular markers were assigned to specific chromosome locations based on a comparison with the homologous genes in the B genome of wheat. Annotation of these genes showed that most had functions related to metabolic processes, hydrolase activity, or catalytic activity. Additionally, we used these markers to identify three wheat-Ae. longissima 1BL•1S^lS translocation lines in somatic variation populations resulting from a cross between wheat cultivar Westonia and a wheat-Ae. longissima substitution line 1S^l(1B). The translocation lines had several low molecular weight glutenin subunits (LMW-GSs) encoding genes beneficial to flour processing quality that came from Ae. longissima 1S^lS. The three translocation lines were also confirmed by genomic in situ hybridization (GISH). These translocation lines will be further evaluated for potential quality improvement of bread-making properties wheat.

　　下载链接：https://link.springer.com/content/pdf/10.1007/s00122-017-2982-5.pdf