Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots.

作者：Li WC, Ji SD, Wang XC, Li ZK, Zhang HC, Tian CZ, Liu YL, Duan CX

影响因子：

刊物名称：Genetics and Molecular Research

出版年份：2015

卷：14  期：2  页码：7258-7266

文章摘要：

Corn DNA was introduced into dry seeds of rice (cv. ‘YuJing-6’) by ion beam irradiation. Proteinase activities in rice seedling roots were subsequently analyzed by renaturation electrophoresis at pH 4.5, 7.0, and 8.5. Proteinase activity was more pronounced on gels at higher pH. Irradiation of rice seedling roots caused the loss of some proteinase bands at all pH conditions although a novel 50-kDa band was found at both pH 7.0 and 8.5. No new proteinase activity was detected at pH 4.5. However, novel bands and bands showing stronger activity were observed at pH 7.0 and 8.5. The data indicate that the expression of proteinases in rice seedling roots was altered following low energy ion beam m