Analysis of promoters of microRNAs from a Glycine max degradome library

作者：Yi-qiang HAN, Zheng HU, Dian-feng ZHENG, Ya-mei GAO

影响因子：

刊物名称：J Zhejiang Univ-Sci B (Biomed & Biotechnol)

出版年份：2014

卷：15  期：2  页码：125-132

文章摘要：

Objective: MicroRNAs (miRNAs) are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes. Our aim was to explore the regulation of miRNA expression. Methods: We used bioinformatics methods to predict the core promoters of 440 miRNAs identified from a soybean (Glycine max) degradome library and to analyze cis-acting elements for 369 miRNAs. Results: The prediction results showed that 83.86% of the 440 miRNAs contained promoters in their upstream sequences, and 8.64% (38 loci) in their downstream sequences. The distributions of two core promoter elements, TATA-boxes and transcription start sites (TSSs), were