Prediction and identification of natural antisense transcripts and their small RNAs in soybean (Glycine max)

作者：Hu Zheng, Jiang Qiyan, Ni Zhiyong and Zhang Hui

影响因子：

刊物名称：BMC Genomics

出版年份：2013

卷：14  期：280  页码：

文章摘要：

Background: Natural antisense transcripts (NATs) are a class of RNAs that contain a sequence complementary to other transcripts. NATs occur widely in eukaryotes and play critical roles in post-transcriptional regulation. Soybean NAT sequences are predicted in the PlantNATsDB, but detailed analyses of these NATs remain to be performed. Results: A total of 26,216 NATs, including 994 cis-NATs and 25,222 trans-NATs, were predicted in soybean. Each sense transcript had 1–177 antisense transcripts. We identified 21 trans-NATs using RT-PCR amplification. Additionally, we identified 179 cis-NATs and 6,629 trans-NATs that gave rise to small RNAs; these were enriched in the NAT overlapping region. The