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Identification and candidate gene analysis of a novel Phytophthora resistance gene Rps10 in a Chinese soybean cultivar

作者:Jiqing Zhang, Changjian Xia, Canxing Duan, Suli Sun, Xiaoming Wang, Xiaofei Wu, Zhendong Zhu

影响因子:

刊物名称:PLoS ONE

出版年份:2013

卷:8  期:7  页码:e69799

文章摘要:

Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2:3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5