Overexpression of gma-MIR394a confers tolerance to drought in transgenic Arabidopsis thaliana

作者：Zhiyong Ni, Zheng Hu, Qiyan Jiang, Hui Zhang

影响因子：

刊物名称：Biochemical and Biophysical Research Communications

出版年份：2012

卷：427  期：  页码：330–335

文章摘要：

MicroRNAs, key posttranscriptional regulators of eukaryotic gene expression, play important roles in plant development and response to stress. In this study, a soybean gma-MIR394a gene was functionally characterized, especially with regard to its role in drought stress resistance. Expression analysis revealed that gma-MIR394a was expressed differentially in various soybean tissues and was induced by drought, high salinity, low temperature stress, and abscisic acid treatment in leaves. One target gene of gma-miR394a, Glyma08g11030, was predicted and verified using a modified 50 RLM-RACE (RNA ligasemediated rapid amplification of 50 cDNA ends) assay. Overexpression of gma-MIR394a resulted in p