Proteomic analysis of ‘hybrid necrosis’ in wheat (Triticum aestivum) leaves

作者：Xinglai Pan, Qiyan Jiang, Qianying Pan, Xuefei Wen, Yinhong Shi, Yongjie Wang, Tianyuan Pan, Sangang Xie, Guiyun Zhang, Shenjie Wu, Yongfeng Cha, Changsheng Zhang, Zongxin Wu, Shihua Shen

影响因子：

刊物名称：Functional Plant Biology

出版年份：2009

卷：36  期：  页码：251-259

文章摘要：

Wheathybridnecrosishasbeengeneticallycharacterisedformanyyears,butthespecificgene(s)andtheprotein products involved in the processes remains unknown. In this study, protein expression in the base (B), mid (M) and tip (T) segmentsoftheFL-2leavesofanecrotichybrid,PZF1anditsparents,Pan555andZheng891,wasanalysedandcompared using a high throughput proteomic approach. Twenty-three protein spots, with significant variations in intensity across the necrotic leaf segments, were analysed by MALDI-TOF-MS, of which, 18 were matched to protein accessions in the NCBI database. Several of these proteins are enzymes involved in the methylation cycle, including AdoHcy hydrolase, AdoMet synthase 3 and methionine synthase 1; AdoHcy hydrolase was downregulated sharply in M and T, and AdoMet synthase 3 and methionine synthase 1 were upregulated gradually from M to T. This result suggests that methylation-associated processes, including epigenetic mechanisms, may play a role in the initiation and development of hybrid necrosis. Several energy cycle-associated proteins and cytoprotective proteins were also differentially expressed across the leaf segments, suggestingtheirdirectassociationwithorpossibleinvolvementinthenecroticprocesses.Thesignificantimbalanceofaheat- shock protein, a transposon protein and a RNA- and ssDNA-binding protein also makes these proteins potential molecular components in the necrotic processes