Identification of MicroRNAs in Wild Soybean (Glycine soja)

作者：Rui Chen, Zheng Hu and Hui Zhang

影响因子：

刊物名称：Journal of Integrative Plant Biology

出版年份：2009

卷：51  期：12  页码：1071–1079

文章摘要：

MicroRNAs (miRNAs) play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified in plants, but no report has been published of wild soybean (Glycine soja Sieb). We constructed a small-RNA library consisting of 2 880 sequences with high quality, in which 1 347 were 19–24 nt in length. By utilizing the miRNA, Rfam and domesticated soybean expressed sequence tag database, we have analyzed and predicted the secondary structure of these small RNAs. As a result, 15 conserved miRNA candidates belonging to eight different families and nine novel miRNA candidates comprising eight families