Crop Germplasm Resources Preservation and Sharing

Team Name: Crop Germplasm Resources Preservation and Sharing

Research Focus: Our team is charged with improving current crop germplasm resources preservation and sharing operations, and our main research interests are:

• Develop methods to preserve species and accessions not currently in the long-term storage, especially cryopreservation in liquid nitrogen for vegetatively propagated germplasm in diverse forms (e.g., shoot tips, dormant buds, pollen);

• Develop methods, especially non-invasive techniques, to improve the efficiency and accuracy in viability, vigor, genetic integrity and potential longevity evaluating of germplasm preserved in low-temperature seed banks, in vitro banks, cryopreservation banks and field banks;

• Explore strategies, theories, techniques and methods for crop germplasm resources in situ and on-farm conservation;

• Develop data standards, databases, application softwares and network information systems for the crop germplasm resources management and research.

Research objectives: Developing strategies and technologies to increase the genetic diversity of germplasm preserved in various facilities, and to improve the effectiveness of crop germplasm genebanks; developing the crop germplasm resource information system to improve the efficiency of germplasm information and accession sharing.

Team Chief Scientist: Dr. LU Xin-xiong

Education Background

Lu Xiniong, an expert in plant genetic resources conservation, received his BSc degree in Plant (1984) from the Xiamen University, and his MS degree in Agronomy (1999) from the Graduate School of Chinese Academy of Agricultural Sciences, P.R. China.

Research Area

He is Head of National Genebank of China, the vice-director of the Conservation and Research Center for Crop Genetic Resources, ICSCAAS, and leader of the Innovation Program of Conservation and Sharing of Crop Genetic Resources at ICSCAAS.

He is majoring in the research and conservation of the national crop genetic resources, both seeds and vegetatively propagated materials. During the last decade, he has been in charge of 12 research projects from the Ministry of Agriculture and Ministry of Science and Technology. He has participated in the organization and implementation of the conservation of over 380 thousands seed accessions at the long-term genebank ofChina, and published 3 technical regulations on collection, management and conservation for crop germplasm resources. He is working on the development of the warning indicators and monitoring methods for viability and the genetic integrity of seeds during conservation, and the establishment of the germplasm information system for safety storage, the study of monitoring, warning and regeneration of conserved germplasm, which provide reliable technical support for the safe conservation and regeneration of germplasm in the National Genebank. He also has excellent experience in anti-ageing studies and genebank construction.  

Publications

He has edited or participated in the editing of 3 books, and has published more than 50 papers as first or corresponding author.

BOOKS

Technical Regulation on Collection for Crop Germplasm Resources (2007)

Technical Regulation on Management for Crop Germplasm Resources (2008)

Technical Regulation on Conservation for Crop Germplasm Resources (2008)

PAPERS

· Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed. Journal of Plant Physiology, 2014,171(2): 140-147 (Corresponding author)

· A real-time, non-invasive, micro-optrode technique for detecting seed viability by using oxygen influx. Scientific Reports, 2013, 3: 3507 (doi: 10.1038/srep03057) (Corresponding author)

· Proteome analysis of maize seeds: the effect of artificial ageing. Physiologia Plantarum, 2011, 2: 126-138 (Corresponding author)

· Effects of different conservation methods on the genetic stability of potato germplasm. Russian Journal of Plant Physiology, 2011, 58(4):728-736 (Corresponding author)

· Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. South African Journal of Botany, 2011, 77 (2): 297-403 (Corresponding author)

· Viability loss of wheat seeds under different storage conditions. Journal of Plant Genetic Resources, 2013, 14(4): 588-593 (In Chinese with English abstract) (Corresponding author)

· Optimal moisture content and physiological bases of rice seeds storage at room temperature. Acta Agronomica Sinica, 2012, 38(9): 1665-1671 (In Chinese with English abstract) (Corresponding author)

· Effect of sample sizes and isolation methods on genetic integrity of Faba Bean (Vicia faba L.) germplasm. Journal of Plant Genetic Resources, 2012, 13(2): 175-181 (In Chinese with English abstract) (Corresponding author)

·Method for identifying Indica-Japonica rice by ising specific heat-stable protein bands. Journal of Plant Genetic Resources, 2011, 12 (1): 19-24 (In Chinese with English abstract) (Corresponding author)

· Germinability and seedling emergence of seeds after 20 years of storage in the National Genebank of China. Journal of Plant Genetic Resources, 2011, 12(6): 934-940 (In Chinese with English abstract) (Corresponding author)

·A rapid extraction method of high quality total RNA from dry seeds. Journal of Plant Genetic Resources, 2010, 11 (3): 360-363. (In Chinese with English abstract) (Corresponding author)

·Effect of seed aging and regeneration times on genetic integrity in soybean “Zhonghuang 18” by SSR markers. Journal of Plant Genetic Resources, 2010, 11 (2): 192-199. (In Chinese with English abstract) (Corresponding author)

· Analysis of viability affecting on genetic integrity in soybean germplasm Zhong Huang 18 by AFLP markers. Acta Agronomica Sinica, 2010, 36 (4): 555-564. (In Chinese with English abstract) (Corresponding author)

·Cryopreservation of in Vitro shoot tips of potato by droplet vitrification and genetic stability of regenerated plantlets. Acta Horticulturae Sinica, 2010, 37(9): 1431-1438 (In Chinese with English abstract) (Corresponding author)

·Elimination of potato virus X and potato spindle tuber viroid by cryopreservation. Molecular Plant Breeding, 2010, 8(3): 605-611 (In Chinese with English abstract) (Corresponding author)

·Cryopreservation of in Vitro shoot-tips of banana (Musa spp.). Journal of Plant Genetic Resources. 2010, 11(1): 34-39 (In Chinese with English abstract) (Corresponding author)

·The optimal moisture content and survival characteristics of rice seeds stored at six climatic zones under room temperature. Chinese Journal of Rice Science, 2009, 23 (6): 621-627. (In Chinese with English abstract) (Corresponding author)

·Research progress on the cryopreservation of sweet potato germplasm. Journal of Plant Genetic Resources, 2009, 10(2): 324-327 (In Chinese with English abstract) (Corresponding author)

·Status quo of crop germplasm resources conservation and sharing system in China. China Science and Technology Resources Review, 2008, 40(4): 20-25 (In Chinese with English abstract) (First author)

·Cryopreservation of shoot tips from Prunus. Acta Horticulturae Sinica, 2008, 35(3): 423-426 (In Chinese with English abstract) (Corresponding author)

· Design and construction of plant germplasm banks. Chinese Bulletin of Botany, 2006, 23 (1): 119-125. (In Chinese with English abstract) (First author)

Academic membership

2011－, Committee of Genetic Resources, Chinese Association of Agricultural Science Societies, executive director

2011－, Committee of Crop Seeds, Crop Science Society of China, vice-chairman

2011－, Committee of Seed Science and Technology, Botanical Society of China, vice-chairman

Team Members: CAO Yong-sheng, YANG Qing-wen, ShI Yun-su, CHEN Xia-ling, FANG Wei, YANG Xin-ming, WANG Lan-fen, XIN Xia