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Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation

  作 者:Jizeng Jia1 *, Shancen Zhao2,3*, Xiuying Kong1 *, Yingrui Li2 *, Guangyao Zhao1 *, Weiming He2 *, Rudi Appels4 *, Matthias Pfeifer5 , Yong Tao2 , Xueyong Zhang1 , Ruilian Jing1 , Chi Zhang2 , Youzhi Ma1 , Lifeng Gao1 , Chuan Gao2 , Manuel Spannagl5 , Klaus F. X. Mayer5 , Dong Li2 , Shengkai Pan2 , Fengya Zheng2,3, Qun Hu6 , Xianchun Xia1 , Jianwen Li2 , Qinsi Liang2 , Jie Chen2 , Thomas Wicker7 , Caiyun Gou2 , Hanhui Kuang6 , Genyun He2 , Yadan Luo2 , Beat Keller7 , Qiuju Xia2 , Peng Lu2 , Junyi Wang2 , Hongfeng Zou2 , Rongzhi Zhang1 , Junyang Xu2 , Jinlong Gao2 , Christopher Middleton7 , Zhiwu Quan2 , Guangming Liu8 , Jian Wang2 , International Wheat Genome Sequencing Consortium{, Huanming Yang2 , Xu Liu1 , Zhonghu He1,9, Long Mao1 & Jun Wang2,10,11

  影响因子:42.315

  刊物名称:Nature

  出版年份:Apr 4, 2013

  doi:10.1038/nature12028

  文章摘要:About 8,000 years ago in the Fertile Crescent, a spontaneous hybridization of the wild diploid grassAegilops tauschii (2n514;DD) with the cultivated tetraploid wheat Triticum turgidum (2n 54x528; AABB) resulted in hexaploid wheat (T. aestivum; 2n56x542; AABBDD).Wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker's flour. Here we describe sequencing the Ae. tauschii genome and obtaining a roughly 90-fold depth of short reads fromlibraries with various insert sizes, to gain a better understanding of this genetically complex plant. The assembled scaffolds represented 83.4% of the genome, of which 65.9% comprised transposable elements. We generated comprehensive RNA-Seq data and used it to identify 43,150 protein-coding genes, of which 30,697 (71.1%) were uniquely anchored to chromosomes with an integrated high-density genetic map. Whole-genome analysis revealed gene family expansion in Ae. tauschii of agronomically relevant gene families that were associated with disease resistance, abiotic stress tolerance and grain quality. This draftgenome sequence provides insight into the environmental adaptation of bread wheat and can aid in defining the large and complicated genomes of wheat species.

  下载链接:https://www.nature.com/articles/nature12028

 



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